Trans-NIH Jak-Stat Initiative - Erythroid: Stat5KO vs WT

Changes in comparison: Test samples for Erythroid: Stat5KO vs WT

Erythropoietic Ter119+ cells from mouse fetal liver cells from Stat5-KO and WT mice.. This used mAdb test: EG1-test-1-FC.

Erythroid: EG001
(T1-)  Stat5KO vs WT --- fold changes < 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[Stat5KO]: EG001-01, EG001-02, EG001-03
   (B) Samples[WT]: EG001-04, EG001-05, EG001-06.

mAdb Microarray Retrieval Report - Test Samples

Preface to table

Row                 A A A B B B  
Row Gene A-B Mean Difference A Mean (Stat5KO) B Mean (WT) A-B p-Value Well ID Feature ID Description EG001-01.Erythroid.Stat5KO.br1.noStim EG001-02.Erythroid.Stat5KO.br2.noStim EG001-03.Erythroid.Stat5KO.br3.noStim EG001-04.Erythroid.WT.br1.noStim EG001-05.Erythroid.WT.br2.noStim EG001-06.Erythroid.WT.br3.noStim Gene Ontology Terms
1 Rsad2 -1.00541 11.49063 12.49605 0.028431841 1337925 1436058_at radical S-adenosyl methionine domain containing 2 (Rsad2), mRNA. 3553.7718 2629.5130 2549.8264 4510.3187 7803.5445 5477.1646
2 Rfk -1.01706 10.22975 11.24681 0.013241846 1327556 1415737_at riboflavin kinase (Rfk), mRNA. 1059.9895 1371.2126 1191.1804 3052.1127 2355.5366 1996.1509
3 4632432E15Rik -1.03215 4.80835 5.84050 0.0042505687 1353617 1432885_at RIKEN cDNA 4632432E15 gene 28.3046 24.6838 31.4849 68.7762 52.9439 51.6702  
4 Rfk -1.03490 10.06125 11.09615 0.020805506 1327927 1416230_at riboflavin kinase (Rfk), mRNA. 1086.4877 1056.3993 1062.5740 2663.5479 2133.3686 1846.2630
5 Socs3 -1.03648 7.14218 8.17866 0.004395647 1328194 1416576_at suppressor of cytokine signaling 3 (Socs3), mRNA. 120.5971 155.6736 150.1349 347.2172 257.0916 272.5061
6 Rsad2 -1.04647 9.58681 10.63328 0.041430984 1331607 1421009_at radical S-adenosyl methionine domain containing 2 (Rsad2), mRNA. 1039.0083 767.2390 570.4251 1238.0962 2215.4173 1460.8151
7   -1.05571 6.81684 7.87255 0.0066383013 1356168 1437087_at Transcribed locus 123.8638 114.2557 101.2511 280.9402 198.3253 231.0111  
8 Trp53bp1 -1.06538 9.29707 10.36245 0.0090267392 1335793 1426957_at transformation related protein 53 binding protein 1 (Trp53bp1), mRNA. 761.2545 526.6445 620.9314 1187.7547 1416.2861 1356.2756
9 Dnaja4 -1.06573 9.86453 10.93026 0.00033938429 1329833 1418591_at DnaJ (Hsp40) homolog, subfamily A, member 4 (Dnaja4), mRNA. 858.1519 967.6132 975.6519 1989.2497 2014.3242 1854.3332
10 Ucp2 -1.06715 9.05346 10.12061 0.017052203 1361995 1444566_at Uncoupling protein 2 (mitochondrial, proton carrier), mRNA (cDNA clone MGC:13955 IMAGE:4205625) 605.6519 591.2656 418.8741 1201.7630 1053.0408 1090.3186
11 Atad4 -1.07729 6.28994 7.36723 0.01279511 1354336 1433870_at ATPase family, AAA domain containing 4 (Atad4), mRNA. 91.6713 76.3957 68.4044 165.3991 164.6009 165.3143  
12 Rragd -1.09951 5.67937 6.77888 0.014578944 1354873 1434909_at Ras-related GTP binding D (Rragd), mRNA. 56.7200 47.7733 49.6660 130.7534 86.4200 117.1841
13 Ctsf -1.10155 8.30660 9.40815 0.015032786 1340886 1451019_at cathepsin F (Ctsf), mRNA. 372.3557 273.5287 311.6344 899.6148 576.9081 604.2813
14 Socs2 -1.10422 4.22756 5.33178 0.044046052 1343687 1418507_s_at Suppressor of cytokine signaling 2 (Socs2), mRNA 17.8044 20.1361 18.3384 49.2846 28.3416 46.7672
15   -1.12764 7.51998 8.64762 0.010980214 1367299 1456204_at Transcribed locus, strongly similar to NP_001118239.1 hypothetical protein LOC440957 [Homo sapiens] 199.8901 170.4654 181.4637 482.2465 326.8741 409.1942  
16 Fgg -1.13757 9.46173 10.59929 0.049718915 1327771 1416025_at fibrinogen gamma chain (Fgg), mRNA. 871.5681 569.6519 706.1201 1579.0987 2254.3835 1048.7630
17 Spata13 -1.17415 8.29268 9.46683 0.0017270212 1342264 1454656_at Spermatogenesis associated 13, mRNA (cDNA clone MGC:170080 IMAGE:8861475) 295.6559 361.7630 288.2847 722.2630 653.6039 750.5664
18 Ghr -1.18131 6.26565 7.44696 0.023284454 1348960 1451501_a_at growth hormone receptor (Ghr), transcript variant 3, mRNA. 95.7515 85.3192 55.7506 190.5392 185.6277 150.1912
19 Dnaja4 -1.20399 8.64099 9.84498 0.0018253591 1329834 1418592_at DnaJ (Hsp40) homolog, subfamily A, member 4 (Dnaja4), mRNA. 363.2998 398.6748 439.2471 799.2239 919.1620 1058.8670
20 Rfk -1.20996 10.05413 11.26410 0.012327788 1327926 1416229_at riboflavin kinase (Rfk), mRNA. 979.6495 1075.5182 1140.5109 2999.7756 2512.5590 1973.7362
21 Clcn2 -1.22520 4.41119 5.63639 0.013266759 1339609 1449248_at chloride channel 2 (Clcn2), mRNA. 20.0007 26.3365 18.2852 42.1835 43.2541 67.4528
22 Spata13 -1.22913 7.46344 8.69257 0.018606018 1356572 1437865_at spermatogenesis associated 13 (Spata13), mRNA. 187.3526 208.9603 140.4213 411.2675 411.1863 418.8038
23 Dnaja4 -1.25760 9.04815 10.30575 0.0026042864 1337468 1434196_at DnaJ (Hsp40) homolog, subfamily A, member 4 (Dnaja4), mRNA. 476.5445 563.7644 552.1969 1272.9513 1238.3512 1286.3662
24 Odc1 -1.25898 11.04641 12.30539 0.0020210188 1346063 1427364_a_at ornithine decarboxylase, structural 1 (Odc1), mRNA. 2469.0588 1833.6796 2089.5172 5094.7275 4581.5947 5555.6635
25 Cd36 -1.29680 5.47789 6.77468 0.0073060718 1340774 1450884_at CD36 antigen, mRNA (cDNA clone MGC:6068 IMAGE:3481681) 39.1388 57.1148 39.5975 98.7144 123.9789 107.2559
26 Gpr150 -1.30245 6.33660 7.63906 0.00096615016 1364787 1447485_at G protein-coupled receptor 150, mRNA (cDNA clone MGC:148214 IMAGE:40107174) 80.4260 92.2921 71.1147 193.4357 179.4297 228.2041
27 Cd82 -1.32889 11.22979 12.55867 0.0033034567 1328061 1416401_at CD82 antigen (Cd82), transcript variant 2, mRNA. 2320.9894 2829.6883 2109.0908 6455.8466 5660.4998 6009.0201
28   -1.33044 4.51723 5.84767 0.025238946 1350770 1428909_at Transcribed locus 31.5752 23.9949 15.8491 46.1837 67.4658 61.2917  
29 Fzd5 -1.39992 7.07836 8.47829 0.0083693082 1366993 1455604_at frizzled homolog 5 (Drosophila) (Fzd5), transcript variant 2, mRNA. 139.7764 163.7443 107.8449 330.3191 287.7759 477.1664
30 Stat5a -1.44942 4.10780 5.55722 0.0062547727 1348627 1450259_a_at signal transducer and activator of transcription 5A (Stat5a), mRNA. 13.8932 16.3582 22.5512 59.7969 39.8737 43.7828
31 Cd36 -1.45049 8.74991 10.20041 0.00050637538 1333195 1423166_at CD36 antigen (Cd36), transcript variant 5, mRNA. 379.3461 486.8553 432.0405 1122.0693 1329.1915 1092.1397
32 Ppm1e -1.53593 3.93365 5.46957 0.0017136379 1354916 1434990_at Protein phosphatase 1E (PP2C domain containing), mRNA (cDNA clone MGC:170530 IMAGE:8861925) 13.9641 17.0712 14.9679 47.5794 36.5826 49.9831
33 Ccrn4l -1.59679 9.03486 10.63165 0.023937466 1345554 1425837_a_at CCR4 carbon catabolite repression 4-like (S. cerevisiae) (Ccrn4l), mRNA. 494.4093 474.9598 614.5408 1407.8149 1181.4982 2400.8203 rhythmic process
34 Gvin1 -1.61572 6.38305 7.99877 0.0082674284 1350962 1429184_at GTPase, very large interferon inducible 1 (Gvin1), transcript variant B, mRNA. 75.2787 66.1066 116.8282 323.1233 265.0227 195.4139
35 Stat5b -1.64926 5.26860 6.91787 0.0018573437 1344534 1422103_a_at signal transducer and activator of transcription 5B (Stat5b), mRNA. 36.8179 32.6977 47.5826 137.0905 121.0724 106.5132
36 Ston2 -1.67702 6.31349 7.99051 0.00096699816 1355327 1435657_at stonin 2 (Ston2), mRNA. 93.0904 66.2764 81.5427 285.4860 215.7075 267.1162
37 Trp53bp1 -1.72283 8.69096 10.41380 0.030032419 1345980 1426956_a_at transformation related protein 53 binding protein 1 (Trp53bp1), mRNA. 660.1404 312.4127 342.2615 1290.7054 1273.4570 1544.4929
38 Cd36 -1.82117 8.36902 10.19019 0.0032191775 1348794 1450883_a_at CD36 antigen (Cd36), transcript variant 5, mRNA. 298.6091 398.8186 303.4571 1145.3741 1232.3186 1129.7630
39   -1.93829 5.09702 7.03531 0.00011176933 1352813 1431742_at Transcribed locus 36.5212 36.3106 30.2337 125.5968 120.8749 148.6645  
40 Tnfrsf21 -2.20578 7.09062 9.29639 0.00013857393 1332863 1422740_at tumor necrosis factor receptor superfamily, member 21 (Tnfrsf21), mRNA. 139.7737 121.2675 149.3812 632.3417 548.6958 716.4694
41 Tnfrsf21 -2.57734 7.76192 10.33926 0.00076077815 1348752 1450731_s_at tumor necrosis factor receptor superfamily, member 21 (Tnfrsf21), mRNA. 179.6531 198.5344 286.7091 1253.8892 1061.2710 1633.7834
42 Socs3 -2.59612 5.38726 7.98338 0.0065395123 1349871 1456212_x_at Suppressor of cytokine signaling 3 (Socs3), mRNA 48.9595 27.6735 54.1104 297.7746 226.1876 240.6310  
43 Socs3 -2.88373 4.68207 7.56579 0.0010702287 1349775 1455899_x_at suppressor of cytokine signaling 3 (Socs3), mRNA. 22.6423 20.6528 36.1766 242.2528 152.9323 183.5801
44 Apol11b -4.20960 3.41062 7.62022 0.00082666764 1371585 1440927_x_at Apolipoprotein L 11b (Apol11b), mRNA 9.9121 9.9725 12.1653 143.1459 227.7468 233.6210  
45 Apol11b -4.64701 3.55236 8.19937 0.0003222647 1368099 1457307_at Apolipoprotein L 11b (Apol11b), mRNA 11.0851 11.0851 13.1410 242.0964 279.7075 375.0408  


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009