Trans-NIH Jak-Stat Initiative - Muscle: Stat5MKO+GH vs WT+GH

Changes in comparison: Test samples for Muscle: Stat5MKO+GH vs WT+GH

Muscle cells from Stat5-null and control 12-week mice with GH treatment. This used mAdb test: EG3.1-test-1-FC.

Muscle: EG003.1
(T1-)  Stat5MKO+GH vs WT+GH --- fold changes < 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[Stat5MKO+GH]: EG003.1-01, EG003.1-02, EG003.1-05
   (B) Samples[WT+GH]: EG003.1-03, EG003.1-04, EG003.1-06.

mAdb Microarray Retrieval Report - Test Samples

Preface to table

Row                 A A A B B B  
Row Gene A-B Mean Difference A Mean (Stat5MKO+GH) B Mean (WT+GH) A-B p-Value Well ID Feature ID Description EG003.1-01.Muscle.Stat5MKO.42.GH EG003.1-02.Muscle.Stat5MKO.43.GH EG003.1-05.Muscle.Stat5MKO.48.GH EG003.1-03.Muscle.WT.44.GH EG003.1-04.Muscle.WT.47.GH EG003.1-06.Muscle.WT.50.GH Gene Ontology Terms
1   -1.00577 4.47558 5.48135 0.00030523845 1363507 1446134_at ESTs 22.3955 23.8650 20.6031 48.0810 41.6238 44.5490  
2 Serpina1c -1.00750 5.86177 6.86927 0.0018003748 1339138 1448680_at serine (or cysteine) peptidase inhibitor, clade A, member 1C (Serpina1c), mRNA. 60.0072 57.7634 56.7346 126.2640 107.7231 117.4833
3 Cited4 -1.00847 8.10305 9.11152 4.3575697e-05 1345355 1425400_a_at Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4, mRNA (cDNA clone IMAGE:3670674) 273.8139 269.4133 281.7780 547.2341 557.8625 554.3976
4 Adamts9 -1.02266 7.42303 8.44569 0.0014195701 1351838 1430352_at MKIAA1312 protein 163.3294 172.5743 179.3186 314.2027 376.0961 358.6830
5 Armcx4 -1.02696 6.47434 7.50130 0.00012462914 1335963 1427167_at Armadillo repeat containing, X-linked 4, mRNA (cDNA clone IMAGE:5374915) 92.0472 89.8266 85.0148 191.7828 181.2917 171.0661  
6   -1.02716 5.66278 6.68994 0.00025077457 1363186 1445809_at Transcribed locus 47.8348 51.0117 53.2824 101.1707 101.9682 106.6829  
7 Dkk3 -1.02749 6.77564 7.80313 0.017327606 1339128 1448669_at dickkopf homolog 3 (Xenopus laevis) (Dkk3), mRNA. 106.7986 110.3035 111.6506 251.0973 183.4840 241.8198
8 Frat2 -1.02764 8.76971 9.79735 7.1981979e-06 1342410 1455220_at frequently rearranged in advanced T-cell lymphomas 2 (Frat2), mRNA. 432.4445 449.9297 427.3303 901.7931 860.0940 908.3186
9 Tob1 -1.03762 9.48397 10.52159 3.4408054e-05 1358662 1440844_at transducer of ErbB-2.1 (Tob1), mRNA. 686.9248 736.2371 726.0295 1542.6583 1455.2829 1414.9135
10 Bdh1 -1.05719 6.16453 7.22172 0.0023140757 1335795 1426959_at 3-hydroxybutyrate dehydrogenase, type 1 (Bdh1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA. 65.4287 77.2778 72.9969 153.1302 145.3725 149.3902
11 Fbxo32 -1.06572 10.25504 11.32077 0.0005490209 1339195 1448747_at F-box protein 32 (Fbxo32), mRNA. 1240.6519 1217.0150 1208.5964 2674.5881 2442.3125 2562.1903
12   -1.06722 4.84376 5.91097 0.0032645792 1357927 1440016_at ESTs 29.1586 28.9029 28.0957 63.2457 54.4033 63.3116  
13 Ucp3 -1.07093 10.49335 11.56428 0.00017987325 1331338 1420657_at uncoupling protein 3 (mitochondrial, proton carrier) (Ucp3), nuclear gene encoding mitochondrial protein, mRNA. 1486.8186 1408.2953 1430.5068 3208.6653 2981.0044 2903.3414
14 Bdh1 -1.07231 8.77250 9.84481 9.6843356e-05 1341716 1452257_at 3-hydroxybutyrate dehydrogenase, type 1 (Bdh1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA. 429.9117 430.9946 451.3442 967.9575 883.5241 909.2381
15 Socs2 -1.08119 8.84492 9.92611 7.1381435e-05 1339494 1449109_at suppressor of cytokine signaling 2 (Socs2), mRNA. 472.4270 433.4186 474.8078 1003.3441 981.8046 934.7482
16 Apbb3 -1.09638 9.49820 10.59459 0.0006813956 1334205 1424457_at Amyloid beta (A4) precursor protein-binding, family B, member 3 (Apbb3), mRNA 740.9057 722.6335 706.3995 1643.5945 1548.8396 1452.3215
17 BC002189 -1.10136 6.77786 7.87922 0.011894786 1341465 1451883_at CDNA sequence BC002189, mRNA (cDNA clone MGC:7386 IMAGE:3487872) 112.4378 108.5944 108.2185 197.3820 258.9297 255.3610  
18   -1.10573 7.86850 8.97423 0.00028336503 1362558 1445148_at Mus musculus 0 day neonate cerebellum cDNA, RIKEN full-length enriched library, clone:C230091K16 product:unknown EST, full insert sequence. 248.1519 219.4084 234.4187 542.2619 507.4418 462.3202  
19 Igh-6 -1.11070 8.46175 9.57245 2.65514e-05 1346060 1427351_s_at Immunoglobulin heavy chain complex, mRNA (cDNA clone MGC:28019 IMAGE:3660622) 334.9651 362.4043 361.0147 774.6081 726.0298 784.7735
20 Gm129 -1.11738 7.35202 8.46939 0.00095723691 1355034 1435188_at predicted gene 129 (Gm129), mRNA. 157.8126 162.8186 169.7019 380.3680 360.8920 324.3706  
21 Neto2 -1.13171 5.28173 6.41344 0.0097901312 1355746 1436309_at neuropilin (NRP) and tolloid (TLL)-like 2 (Neto2), mRNA. 30.9623 42.6913 44.5354 94.6231 83.2493 78.6205
22 Gpr177 -1.13778 7.14735 8.28513 0.0033455627 1333713 1423825_at G protein-coupled receptor 177 (Gpr177), mRNA 139.0409 144.9107 141.4038 338.6226 318.3671 281.5625
23 Apold1 -1.13785 7.39455 8.53240 0.00011455178 1359017 1441228_at Apolipoprotein L domain containing 1 (Apold1), mRNA 166.6573 176.0408 162.3692 396.8003 359.5980 355.7399  
24 Ppargc1a -1.13858 6.95255 8.09113 0.00093133607 1342923 1460336_at peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1, mRNA. 135.3742 125.2048 112.1038 269.4157 288.4518 260.9304
25 Casp12 -1.14336 7.44898 8.59234 0.00050990983 1330166 1418981_at caspase 12 (Casp12), mRNA. 164.7075 175.9609 184.0654 413.8376 349.4793 397.5633
26 Amd1 -1.14668 11.55101 12.69769 7.1412985e-05 1343385 1416835_s_at S-adenosylmethionine decarboxylase 1 (Amd1), mRNA. 2995.0112 2961.6519 3045.5934 6635.0619 6660.7491 6634.2451
27   -1.15584 4.75702 5.91286 0.013258111 1352872 1431869_at Transcribed locus 24.0320 31.9706 25.7320 74.0188 64.2147 46.0115  
28 Rgs2 -1.16736 7.61139 8.77875 0.00066732964 1372117 1447830_s_at regulator of G-protein signaling 2 (Rgs2), mRNA. 195.1244 198.8168 192.7568 462.8211 440.0609 415.9858
29 Zfp462 -1.16884 6.29959 7.46844 0.00016520924 1367622 1456789_at zinc finger protein 462 (Zfp462), mRNA. 72.7277 79.6896 84.3333 165.3645 186.3418 180.2684
30   -1.18391 5.15256 6.33647 0.0014605726 1353251 1432461_at Transcribed locus 31.3186 35.9796 39.9359 84.3463 74.2800 84.2294  
31 Ttr -1.19392 3.35731 4.55123 0.0020615281 1349780 1455913_x_at transthyretin (Ttr), mRNA. 10.0928 8.9278 11.9452 24.0086 26.5427 20.2236
32 Gramd1a -1.19860 8.64527 9.84388 4.1399609e-06 1343360 1416708_a_at GRAM domain containing 1A (Gramd1a), mRNA. 407.9903 406.7446 386.8004 942.3607 894.1517 921.0368
33 Ttr -1.19950 4.35913 5.55863 0.004162931 1349572 1454608_x_at transthyretin (Ttr), mRNA. 22.9533 20.3788 18.4784 57.4732 44.0718 41.3349
34 Inhbb -1.20941 6.38489 7.59430 0.00050009481 1335714 1426858_at Inhibin beta-B subunit 78.5539 87.4236 84.9827 212.5964 191.2307 177.5100
35 2310003H01Rik -1.21346 7.18196 8.39542 0.00022867201 1365491 1453368_at RIKEN cDNA 2310003H01 gene, mRNA (cDNA clone MGC:37540 IMAGE:4986832) 134.4837 144.7839 157.2401 360.5110 306.5686 345.4387
36 Tsc22d1 -1.21729 4.04999 5.26728 0.030392382 1364676 1447360_at TSC22-related inducible leucine zipper 1b (Tilz1b) 13.8693 22.9534 14.2759 28.9957 55.7832 35.3177
37 Tcfcp2l1 -1.23042 7.82647 9.05689 0.00013744918 1329435 1418091_at transcription factor CP2-like 1 (Tcfcp2l1), mRNA. 243.0805 216.2775 222.4566 550.9603 512.3839 535.1430
38 Mras -1.24367 6.71447 7.95814 0.012297776 1348434 1449590_a_at muscle and microspikes RAS (Mras), mRNA. 108.9853 107.4891 98.8646 311.8064 219.6831 224.5103
39   -1.24403 6.22486 7.46889 0.00025802251 1364374 1447023_at ESTs 71.0820 80.9267 72.7367 173.5964 195.1596 164.1151  
40 9130404H23Rik -1.26640 5.32932 6.59572 0.011571678 1368912 1458210_at PREDICTED: Mus musculus RIKEN cDNA 9130404H23 gene (9130404H23Rik), mRNA 42.0233 40.3867 38.2933 118.1431 94.5930 80.9575  
41 Casp12 -1.27366 6.69284 7.96650 0.00012121675 1339649 1449297_at caspase 12 (Casp12), mRNA. 101.0482 111.2058 98.5324 258.5023 239.1447 253.1287
42 Igh-6 -1.28640 10.37595 11.66235 9.9677398e-05 1346052 1427329_a_at Immunoglobulin heavy chain complex, mRNA (cDNA clone MGC:28019 IMAGE:3660622) 1345.0827 1318.0969 1323.4778 3270.1303 3132.1231 3324.6294
43 Klf15 -1.29186 10.11271 11.40456 0.00042828507 1338746 1448181_at Kruppel-like factor 15 (Klf15), mRNA. 1050.2375 1121.8854 1151.9891 2694.9050 2752.8130 2685.5082
44 Arrdc2 -1.29315 9.47776 10.77091 0.0016019058 1350443 1428352_at arrestin domain containing 2 (Arrdc2), mRNA. 721.2315 703.3000 714.5902 1895.7038 1694.9889 1660.2075  
45 Acsl3 -1.30294 7.30039 8.60333 0.00064747098 1372229 1452771_s_at acyl-CoA synthetase long-chain family member 3 (Acsl3), transcript variant 3, mRNA. 163.2630 149.6342 160.3225 431.9551 370.4974 367.5856
46 Kcnf1 -1.32182 6.63304 7.95486 0.00014807324 1359079 1441300_at Potassium voltage-gated channel, subfamily F, member 1 (Kcnf1), mRNA 107.1100 91.2041 100.0896 248.2030 229.3167 268.3572
47 Cd28 -1.37695 5.80231 7.17926 0.00020118959 1338084 1437025_at CD28 antigen (Cd28), mRNA. 51.9557 61.4238 54.4551 152.8239 135.7862 146.7140
48 Stat5a -1.40167 4.88226 6.28392 0.000711184 1348627 1450259_a_at signal transducer and activator of transcription 5A (Stat5a), mRNA. 25.8331 31.5116 31.5116 82.9659 78.6861 72.4687
49 Fbxo32 -1.40226 9.26325 10.66551 6.1049745e-05 1328966 1417522_at F-box protein 32 (Fbxo32), mRNA. 615.6347 623.0472 604.9297 1620.7427 1626.4259 1625.4179
50 Socs2 -1.40391 7.99067 9.39457 6.3920059e-05 1343687 1418507_s_at Suppressor of cytokine signaling 2 (Socs2), mRNA 248.9299 268.4852 246.2027 653.5010 643.1115 725.4607
51 Slc25a25 -1.46113 9.95830 11.41944 4.1933681e-06 1334422 1424735_at solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 (Slc25a25), nuclear gene encoding mitochondrial protein, mRNA. 953.5615 1016.9887 1015.2672 2841.0170 2711.1166 2667.8203
52   -1.46205 7.10259 8.56464 0.000107031 1360668 1443100_at Transcribed locus 140.4297 146.9383 125.8017 364.9948 405.3497 366.8801  
53 Dkk3 -1.48988 6.40382 7.89370 0.00013983873 1328794 1417312_at dickkopf homolog 3 (Xenopus laevis) (Dkk3), mRNA. 76.3389 89.8983 88.4559 262.7482 224.6651 227.8496
54 Slc7a8 -1.49984 7.96062 9.46046 0.00020570579 1329298 1417929_at solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 (Slc7a8), mRNA. 246.7763 268.5551 233.2480 630.5303 733.3004 756.2393
55 Tm6sf2 -1.51615 5.36625 6.88239 0.0030043878 1334673 1425079_at transmembrane 6 superfamily member 2 (Tm6sf2), mRNA. 47.3997 39.6747 37.3176 120.2125 113.8210 120.0190
56 Stat5b -1.55482 7.49408 9.04890 0.00031706532 1344533 1422102_a_at signal transducer and activator of transcription 5B (Stat5b), mRNA. 200.1609 167.7075 174.5408 560.8443 521.5580 507.9575
57 Chd6 -1.55773 6.99415 8.55188 0.00030105915 1336133 1427384_at Chromodomain helicase DNA binding protein 6, mRNA (cDNA clone IMAGE:3597702) 132.7359 136.8830 114.0280 336.8695 369.3644 424.8182
58 Gadl1 -1.58941 6.83063 8.42004 0.0054594723 1359154 1441384_at Glutamate decarboxylase-like 1 (Gadl1), mRNA 114.4313 113.9699 113.0672 304.5925 328.7047 401.3536
59   -1.60369 7.91103 9.51472 0.00074085621 1369075 1458409_at ESTs 255.0898 229.1534 238.5366 647.8549 746.7632 809.0697  
60 Ddit4 -1.70136 11.00698 12.70834 5.2878803e-06 1336689 1428306_at DNA-damage-inducible transcript 4 (Ddit4), mRNA. 2022.3901 2068.3186 2083.5964 6563.1989 6610.9871 6908.7095
61 LOC100048105 -1.72338 6.23069 7.95408 0.00050866253 1335161 1425965_at PREDICTED: similar to Ubc protein, transcript variant 2 (LOC100048105), mRNA. 73.1578 76.2143 75.9593 269.5804 235.2393 240.4633  
62 Cish -1.85801 10.20218 12.06020 2.3745585e-06 1339177 1448724_at cytokine inducible SH2-containing protein (Cish), mRNA. 1230.2147 1169.5646 1136.2792 4293.6797 4113.7206 4409.4028
63 Aldh9a1 -1.98268 7.97343 9.95611 8.6036817e-05 1347565 1437398_a_at aldehyde dehydrogenase 9, subfamily A1 (Aldh9a1), mRNA. 237.6797 244.9858 272.6434 1031.1579 993.4510 956.7375
64 Aldh9a1 -1.99216 9.07099 11.06315 0.00018455543 1347360 1436689_a_at aldehyde dehydrogenase 9, subfamily A1 (Aldh9a1), mRNA. 549.7658 560.6155 504.7480 2143.1672 2100.7630 2175.6269
65 Mafb -2.00842 9.43076 11.43918 8.6868417e-05 1341365 1451716_at V-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), mRNA 715.7394 690.2579 665.3594 2766.2138 2808.5714 2755.7235
66 Cd28 -2.07162 4.76589 6.83751 0.0045513571 1329031 1417597_at CD28 antigen (Cd28), mRNA. 26.8275 24.5152 30.6207 152.6904 106.3417 92.1240
67 Pnmt -2.12516 6.92598 9.05114 6.0248296e-05 1340562 1450606_at phenylethanolamine-N-methyltransferase (Pnmt), mRNA. 119.8420 114.0869 131.5029 478.6041 582.4354 535.5104
68 Stat5b -2.23420 7.57364 9.80783 5.4362387e-05 1344534 1422103_a_at signal transducer and activator of transcription 5B (Stat5b), mRNA. 195.1879 183.1424 193.3896 842.1944 887.5067 963.3258
69   -2.25013 8.31204 10.56217 0.00037745141 1342536 1455869_at Transcribed locus, moderately similar to XP_001477892.1 PREDICTED: hypothetical protein [Mus musculus] 323.9602 308.2770 321.4307 1664.2896 1416.2154 1466.3313  
70 Slc15a5 -2.26503 4.41609 6.68113 0.0014649411 1336340 1427722_at solute carrier family 15, member 5 (Slc15a5), mRNA. 20.9009 20.5427 22.6625 93.2300 94.2457 122.9824
71 Mafb -2.28473 8.49461 10.77934 9.287748e-06 1341364 1451715_at v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), mRNA. 351.4852 390.0426 342.2789 1629.9509 1790.5964 1860.1253
72   -2.39750 8.06657 10.46408 2.9695035e-06 1346626 1431213_a_at Transcribed locus, weakly similar to XP_001379802.1 PREDICTED: similar to pol protein [Monodelphis domestica] 284.7408 257.7510 262.5376 1491.9889 1412.7241 1337.1505  
73   -2.43276 6.00594 8.43870 4.5809926e-05 1369924 1459289_at Mus musculus 16 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:9630027C04 product:unknown EST, full insert sequence. 58.4382 71.7892 63.2632 322.4894 345.6549 374.7537  
74 Gdpd3 -2.57115 6.08938 8.66052 5.1116691e-07 1348421 1449526_a_at glycerophosphodiester phosphodiesterase domain containing 3 (Gdpd3), mRNA. 69.0098 69.3379 65.9741 393.3762 400.2617 420.8046
75 Kcnf1 -2.75248 5.74620 8.49868 6.0764879e-05 1366492 1454768_at potassium voltage-gated channel, subfamily F, member 1 (Kcnf1), mRNA. 56.6816 47.6452 57.2635 379.9297 338.0776 368.4297
76 1700001O22Rik -2.98010 5.76955 8.74965 0.00018664134 1345143 1424795_a_at RIKEN cDNA 1700001O22 gene (1700001O22Rik), mRNA. 67.1359 51.3696 47.0724 489.9384 431.6515 377.0922  


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009