Trans-NIH Jak-Stat Initiative - Muscle: Stat5MKO-GH vs WT-GH

Changes in comparison: Test samples for Muscle: Stat5MKO-GH vs WT-GH

Muscle cells from Stat5-null and control 15-week mice without GH treatment. This used mAdb test: EG3.2-test-1+FC.

Muscle: EG003.2
(T2+)  Stat5MKO-GH vs WT-GH --- fold changes > 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[Stat5MKO-GH]: EG003.2-04, EG003.2-05, EG003.2-06
   (B) Samples[WT-GH]: EG003.2-01, EG003.2-02, EG003.2-03.

mAdb Microarray Retrieval Report - Test Samples

Preface to table

Row                 A A A B B B  
Row Gene A-B Mean Difference A Mean (Stat5MKO-GH) B Mean (WT-GH) A-B p-Value Well ID Feature ID Description EG003.2-01.Muscle.WT.4038WT.noStim EG003.2-02.Muscle.WT.4060WT.noStim EG003.2-03.Muscle.WT.4063WT.noStim EG003.2-04.Muscle.Stat5MKO.4035KO.noStim EG003.2-05.Muscle.Stat5MKO.4036KO.noStim EG003.2-06.Muscle.Stat5MKO.4042KO.noStim Gene Ontology Terms
1 1700001O22Rik 2.89579 9.40274 6.50696 0.0010788846 1345143 1424795_a_at RIKEN cDNA 1700001O22 gene (1700001O22Rik), mRNA. 555.6433 734.1229 760.2554 124.9346 74.9565 80.3294  
2 Stat5b 2.72965 10.48005 7.75040 0.0066651671 1344534 1422103_a_at signal transducer and activator of transcription 5B (Stat5b), mRNA. 1420.4606 1362.1924 1505.7619 301.7720 176.5317 187.4154
3 Cish 2.51205 10.82937 8.31732 0.016365591 1339177 1448724_at cytokine inducible SH2-containing protein (Cish), mRNA. 1080.4852 1721.8420 3238.0171 495.1049 180.4638 363.2432
4 Socs2 2.06717 8.67601 6.60884 0.011818782 1343687 1418507_s_at Suppressor of cytokine signaling 2 (Socs2), mRNA 255.1171 453.8417 590.9852 134.6512 73.0052 94.5830
5 Kcnf1 2.05301 6.62131 4.56831 0.0235545 1366492 1454768_at potassium voltage-gated channel, subfamily F, member 1 (Kcnf1), mRNA. 68.6281 77.4844 179.4429 28.0236 16.7551 28.4394
6 Socs2 1.95466 9.64328 7.68861 0.0059788138 1339494 1449109_at suppressor of cytokine signaling 2 (Socs2), mRNA. 597.6048 845.6000 1011.9679 231.8657 185.9429 203.6540
7 Gadl1 1.85758 8.98819 7.13061 0.049384706 1359154 1441384_at Glutamate decarboxylase-like 1 (Gadl1), mRNA 458.4861 460.1643 620.7335 270.0419 105.7489 96.3556
8 Slc15a5 1.80022 5.78354 3.98332 0.044181429 1336340 1427722_at solute carrier family 15, member 5 (Slc15a5), mRNA. 106.4931 42.3269 37.0779 16.8811 9.8637 23.7602
9 Casp12 1.76163 8.34531 6.58368 0.0032759493 1339649 1449297_at caspase 12 (Casp12), mRNA. 298.3186 317.9297 362.7039 116.8754 77.1682 97.8343
10   1.62330 9.19177 7.56847 0.0031722308 1341594 1452106_at Transcribed locus 558.7154 478.8456 747.4913 220.8808 192.5369 160.8191  
11 Casp12 1.60060 9.02264 7.42204 0.00052098409 1330166 1418981_at caspase 12 (Casp12), mRNA. 502.3105 462.2016 605.9685 176.4864 150.8865 189.4122
12   1.56591 6.58643 5.02052 0.0076407575 1349197 1452107_s_at Transcribed locus 107.3401 76.1409 108.5822 32.5923 30.6299 34.2547  
13 Cd28 1.56502 6.38087 4.81585 0.027523777 1329031 1417597_at CD28 antigen (Cd28), mRNA. 55.3328 113.4937 92.1606 32.1468 27.0840 25.6622
14 Stat5b 1.56308 9.30647 7.74340 0.0012332287 1344533 1422102_a_at signal transducer and activator of transcription 5B (Stat5b), mRNA. 700.2908 558.4112 649.1553 224.4297 204.4297 214.4663
15 Acsl3 1.55931 8.37004 6.81073 0.0051952203 1372229 1452771_s_at acyl-CoA synthetase long-chain family member 3 (Acsl3), transcript variant 3, mRNA. 282.7366 290.2828 441.2631 145.0960 98.2182 99.2781
16 Mafb 1.42277 9.62010 8.19733 0.018186563 1341365 1451716_at V-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), mRNA 652.5212 666.6209 1120.3174 341.1308 278.8834 265.8186
17 Dkk3 1.41189 7.66216 6.25027 0.019000024 1328794 1417312_at dickkopf homolog 3 (Xenopus laevis) (Dkk3), mRNA. 157.9852 183.0014 287.4398 85.9731 78.4342 65.4170
18 Mafb 1.40745 8.24774 6.84029 0.024961307 1341364 1451715_at v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb), mRNA. 229.7631 276.4024 442.2075 129.3800 117.5628 98.9141
19 Socs2 1.31611 6.59017 5.27405 0.0075799986 1356918 1438470_at Suppressor of cytokine signaling 2, mRNA (cDNA clone IMAGE:1068260) 73.7221 105.0405 115.4938 36.9443 33.9261 46.2234
20 Neto2 1.23588 7.12328 5.88740 0.0088415993 1355746 1436309_at neuropilin (NRP) and tolloid (TLL)-like 2 (Neto2), mRNA. 128.8303 133.1761 157.9499 75.2886 49.8386 55.2788
21 Stat5a 1.21721 7.23537 6.01816 0.0019973238 1348627 1450259_a_at signal transducer and activator of transcription 5A (Stat5a), mRNA. 166.3186 137.2517 149.8765 74.5429 65.4503 55.7989
22   1.15876 9.88899 8.73023 0.0024023272 1367020 1455647_at Transcribed locus 885.6439 1075.1794 895.1835 502.6790 403.8789 377.2622  
23 Gadl1 1.13957 7.49263 6.35306 0.02749863 1352211 1430843_at glutamate decarboxylase-like 1 (Gadl1), mRNA. 179.3990 163.5236 199.1235 104.1016 60.4963 86.7366
24 Polk 1.13053 9.04485 7.91431 0.0030263012 1339803 1449483_at polymerase (DNA directed), kappa (Polk), mRNA. 465.5213 505.1972 626.4852 266.3712 204.3848 257.8708
25 Odc1 1.13024 10.73701 9.60678 0.033244858 1347648 1437711_x_at ornithine decarboxylase, structural 1 (Odc1), mRNA. 1810.1088 1270.1519 2162.3660 1041.4852 824.2759 552.1519
26 B130066H01Rik 1.12609 5.18662 4.06053 0.013339111 1363875 1446509_at RIKEN cDNA B130066H01 gene 35.0984 31.0789 44.2829 13.2299 21.4879 16.3409  
27   1.12090 7.84839 6.72749 0.0068605237 1354351 1433898_at Transcribed locus 246.9030 184.4152 268.8305 125.0574 94.8466 100.3231  
28 Armcx4 1.11470 7.44107 6.32637 0.048187743 1335963 1427167_at Armadillo repeat containing, X-linked 4, mRNA (cDNA clone IMAGE:5374915) 178.8125 152.7356 192.1384 116.8197 74.4440 59.4200  
29   1.09288 8.39993 7.30705 0.04783182 1372388 1455966_s_at Transcribed locus 354.7032 306.4024 354.5955 218.0023 159.4280 114.2602  
30 Mchr1 1.07710 7.73639 6.65929 0.0034490492 1336547 1428031_at melanin-concentrating hormone receptor 1 (Mchr1), mRNA. 200.7433 217.3496 222.2571 107.0690 87.7749 109.8771
31 Acsl3 1.07367 5.43131 4.35764 0.0025226076 1350458 1428387_at acyl-CoA synthetase long-chain family member 3 (Acsl3), transcript variant 3, mRNA. 43.8126 40.1095 45.7212 23.6600 19.1201 19.0474
32 Odc1 1.06232 10.55471 9.49239 0.020605427 1346063 1427364_a_at ornithine decarboxylase, structural 1 (Odc1), mRNA. 1682.9176 1113.2225 1816.3872 845.2527 743.7337 594.4004
33 9130404H23Rik 1.04192 6.25963 5.21771 0.034362544 1368912 1458210_at PREDICTED: Mus musculus RIKEN cDNA 9130404H23 gene (9130404H23Rik), mRNA 69.7181 70.0735 92.0686 34.4037 28.8514 51.9150  
34 Odc1 1.02476 12.35765 11.33289 0.022428023 1347907 1438761_a_at ornithine decarboxylase, structural 1 (Odc1), mRNA. 5555.4619 3957.2088 6576.0122 2995.5964 2816.5713 2034.2963
35 Slc30a2 1.00905 8.54255 7.53349 0.00048567467 1336101 1427339_at Solute carrier family 30 (zinc transporter), member 2, mRNA (cDNA clone IMAGE:4239648) 359.7200 350.5555 411.1179 201.9090 174.3739 180.6281  
36 8430426J06Rik 1.00876 8.45093 7.44217 0.048013374 1357769 1439844_at CDNA clone IMAGE:3368992 252.3677 374.0326 453.9504 160.1131 175.2817 187.4064  


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009