Trans-NIH Jak-Stat Initiative - Muscle: Stat5MKO-GH vs WT-GH

Changes in comparison: Test samples for Muscle: Stat5MKO-GH vs WT-GH

Muscle cells from Stat5-null and control 15-week mice without GH treatment. This used mAdb test: EG3.2-test-1-FC.

Muscle: EG003.2
(T2-)  Stat5MKO-GH vs WT-GH --- fold changes < 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[Stat5MKO-GH]: EG003.2-04, EG003.2-05, EG003.2-06
   (B) Samples[WT-GH]: EG003.2-01, EG003.2-02, EG003.2-03.

mAdb Microarray Retrieval Report - Test Samples

Preface to table

Row                  
Row Gene A-B Mean Difference A Mean (Stat5MKO-GH) B Mean (WT-GH) A-B p-Value Well ID Feature ID Description Gene Ontology Terms
1 Ak3l1 -1.05022 6.48540 7.53562 0.0093021397 1332186 1421830_at adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA.
2 Mustn1 -1.06881 10.41685 11.48566 0.018573107 1335990 1427201_at musculoskeletal, embryonic nuclear protein 1 (Mustn1), mRNA. nucleus
3 Tnfaip2 -1.08163 10.37704 11.45867 0.040723537 1347930 1438855_x_at tumor necrosis factor, alpha-induced protein 2  
4 Serpina1c -1.14894 4.84929 5.99823 0.010746921 1339138 1448680_at serine (or cysteine) peptidase inhibitor, clade A, member 1C (Serpina1c), mRNA.
5 Stat3 -1.15172 9.22101 10.37272 0.0053308228 1343080 1460700_at signal transducer and activator of transcription 3 (Stat3), transcript variant 3, mRNA.
6 Serpinb1a -1.22031 6.14677 7.36708 0.00034890631 1327998 1416318_at serine (or cysteine) peptidase inhibitor, clade B, member 1a (Serpinb1a), mRNA.
7 Ak3l1 -1.22369 7.22808 8.45177 0.040083447 1348663 1450387_s_at adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA.
8 Sln -1.23119 7.68650 8.91769 0.0054222097 1331506 1420884_at sarcolipin (Sln), mRNA.
9   -1.24487 7.35232 8.59718 0.0037471724 1367914 1457102_at Transcribed locus  
10 Gamt -1.24753 9.20233 10.44986 0.0052749455 1332719 1422558_at guanidinoacetate methyltransferase (Gamt), mRNA.
11 Tnfaip2 -1.34592 9.55423 10.90015 0.019383957 1327963 1416273_at tumor necrosis factor, alpha-induced protein 2 (Tnfaip2), mRNA.
12 Fgfbp1 -1.41894 5.16466 6.58361 0.0011928135 1330250 1419086_at fibroblast growth factor binding protein 1 (Fgfbp1), mRNA.
13   -1.43316 7.74730 9.18046 0.020322115 1365310 1453119_at Transcribed locus  
14 1200009I06Rik -2.05051 7.53553 9.58603 0.016057694 1370826 1428420_a_at RIKEN cDNA 1200009I06 gene (1200009I06Rik), mRNA.  
15 Myh7 -2.16573 7.41775 9.58347 0.049555307 1348252 1448554_s_at myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7), mRNA.
16 Tpm3 -2.38939 8.57935 10.96874 0.035580778 1340138 1449997_at Tropomyosin 3, gamma, mRNA (cDNA clone MGC:36031 IMAGE:4502880)
17 Tnni1 -2.40297 7.86608 10.26905 0.028790526 1348770 1450813_a_at troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA.
18   -2.43608 5.03578 7.47186 0.021004874 1368821 1458115_at Transcribed locus  
19 Tnnc1 -2.54042 8.50746 11.04788 0.040945566 1329660 1418370_at troponin C, cardiac/slow skeletal (Tnnc1), mRNA.


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009