Trans-NIH Jak-Stat Initiative - Muscle: Stat5MKO-GH vs WT-GH

Changes in comparison: Test samples for Muscle: Stat5MKO-GH vs WT-GH

Muscle cells from Stat5-null and control 15-week mice without GH treatment. This used mAdb test: EG3.2-test-1-FC.

Muscle: EG003.2
(T2-)  Stat5MKO-GH vs WT-GH --- fold changes < 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[Stat5MKO-GH]: EG003.2-04, EG003.2-05, EG003.2-06
   (B) Samples[WT-GH]: EG003.2-01, EG003.2-02, EG003.2-03.

mAdb Microarray Retrieval Report - Test Samples

Preface to table

Row                 A A A B B B  
Row Gene A-B Mean Difference A Mean (Stat5MKO-GH) B Mean (WT-GH) A-B p-Value Well ID Feature ID Description EG003.2-01.Muscle.WT.4038WT.noStim EG003.2-02.Muscle.WT.4060WT.noStim EG003.2-03.Muscle.WT.4063WT.noStim EG003.2-04.Muscle.Stat5MKO.4035KO.noStim EG003.2-05.Muscle.Stat5MKO.4036KO.noStim EG003.2-06.Muscle.Stat5MKO.4042KO.noStim Gene Ontology Terms
1 Ak3l1 -1.05022 6.48540 7.53562 0.0093021397 1332186 1421830_at adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA. 113.1034 81.5462 77.9860 162.8186 218.5802 179.4852
2 Mustn1 -1.06881 10.41685 11.48566 0.018573107 1335990 1427201_at musculoskeletal, embryonic nuclear protein 1 (Mustn1), mRNA. 1112.3910 1549.3411 1482.3400 3852.3579 2425.2815 2524.0403 nucleus
3 Tnfaip2 -1.08163 10.37704 11.45867 0.040723537 1347930 1438855_x_at tumor necrosis factor, alpha-induced protein 2 1937.2358 1153.9852 1051.9975 2594.0235 3370.5477 2549.9669  
4 Serpina1c -1.14894 4.84929 5.99823 0.010746921 1339138 1448680_at serine (or cysteine) peptidase inhibitor, clade A, member 1C (Serpina1c), mRNA. 35.0984 23.1433 29.4871 67.2878 68.1489 56.9569
5 Stat3 -1.15172 9.22101 10.37272 0.0053308228 1343080 1460700_at signal transducer and activator of transcription 3 (Stat3), transcript variant 3, mRNA. 563.5964 511.8688 736.6675 1215.0369 1560.5489 1229.2415
6 Serpinb1a -1.22031 6.14677 7.36708 0.00034890631 1327998 1416318_at serine (or cysteine) peptidase inhibitor, clade B, member 1a (Serpinb1a), mRNA. 74.1940 72.3079 66.3027 182.2095 154.0949 160.2428
7 Ak3l1 -1.22369 7.22808 8.45177 0.040083447 1348663 1450387_s_at adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA. 218.2075 134.3381 114.9569 315.7167 362.3709 375.1926
8 Sln -1.23119 7.68650 8.91769 0.0054222097 1331506 1420884_at sarcolipin (Sln), mRNA. 178.8438 211.0753 231.5755 587.5316 395.1282 487.1988
9   -1.24487 7.35232 8.59718 0.0037471724 1367914 1457102_at Transcribed locus 195.5695 138.2218 161.4077 393.0408 457.7161 322.8464  
10 Gamt -1.24753 9.20233 10.44986 0.0052749455 1332719 1422558_at guanidinoacetate methyltransferase (Gamt), mRNA. 517.6892 585.7949 674.0960 1349.6060 1406.0643 1441.9590
11 Tnfaip2 -1.34592 9.55423 10.90015 0.019383957 1327963 1416273_at tumor necrosis factor, alpha-induced protein 2 (Tnfaip2), mRNA. 1091.3115 612.3186 635.9214 1565.4321 2320.3607 1921.4254
12 Fgfbp1 -1.41894 5.16466 6.58361 0.0011928135 1330250 1419086_at fibroblast growth factor binding protein 1 (Fgfbp1), mRNA. 30.1154 38.2075 40.1069 111.3767 89.7235 88.2856
13   -1.43316 7.74730 9.18046 0.020322115 1365310 1453119_at Transcribed locus 210.8462 215.7630 218.0549 459.4518 757.4940 561.2626  
14 1200009I06Rik -2.05051 7.53553 9.58603 0.016057694 1370826 1428420_a_at RIKEN cDNA 1200009I06 gene (1200009I06Rik), mRNA. 208.0928 181.2786 169.2994 576.1519 675.4474 1166.6011  
15 Myh7 -2.16573 7.41775 9.58347 0.049555307 1348252 1448554_s_at myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7), mRNA. 98.3841 328.7075 154.5816 769.0408 813.4041 721.9121
16 Tpm3 -2.38939 8.57935 10.96874 0.035580778 1340138 1449997_at Tropomyosin 3, gamma, mRNA (cDNA clone MGC:36031 IMAGE:4502880) 249.1013 748.7493 300.0638 1856.5197 1897.4333 2285.0254
17 Tnni1 -2.40297 7.86608 10.26905 0.028790526 1348770 1450813_a_at troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA. 146.8151 436.8152 198.0208 1158.1453 1100.0280 1474.7292
18   -2.43608 5.03578 7.47186 0.021004874 1368821 1458115_at Transcribed locus 31.6866 34.7647 32.0440 106.5041 232.3178 226.1078  
19 Tnnc1 -2.54042 8.50746 11.04788 0.040945566 1329660 1418370_at troponin C, cardiac/slow skeletal (Tnnc1), mRNA. 269.9078 781.6517 228.4423 2008.4111 1945.6615 2428.3396


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009