Trans-NIH Jak-Stat Initiative - Muscle: WT+GH vs WT-GH

Changes in comparison: Test samples for Muscle: WT+GH vs WT-GH

Muscle cells from WT 12-week mice with GH treatment and 15 week WT without GT treatment. This used mAdb test: EG3.1-test-3+FC.

Muscle: EG003.1+EG003.2
(T4+)  WT+GH vs WT-GH --- fold changes > 2X and p-Value<0.05.

This mAdb-derived analysis report compares two groups of samples (A and B) using the paired t-test followed by a fold-change test with the threshold values listed above. The t-test is only done if are at least two samples/condition. It performs fold-change thresholding in either case. The mean A and mean B values are shown as log2 values in the following table as A Mean and B Mean. If there are not enough samples in either condition A or condition B, it only compares the fold-change (mean A/mean B) computed as the difference between the log2 means shown as A-B Mean Difference in this report - see Data Analysis Methods for more discussion. If the t-test is performed, the p-Value is reported as A-B p-Value. Individual sample expression values are reported as the RMA normalized values not using the log2. The tests, thresholding and mAdb Retrieval Report were generated on the mAdb system for the GSP data and subsequently converted to this Web page.

Clicking on the table entries for Gene, Well ID, and Feature ID will go to the GeneCard, mAdb Feature Report, and Affymetrix probe database Web page for that entry. The genes are sorted by A-B Mean Difference with:
   (A) Samples[WT+GH]: EG003.1-03, EG003.1-04, EG003.1-06
   (B) Samples[WT-GH]: EG003.2-01, EG003.2-02, EG003.2-03.

$$LIMITED-ROWS$$

Preface mAdb Microarray Retrieval Report - Test Samples

  A 1 02-09-07_Hennighausen_PK_Mouse430_2_M44 GSP-EG003.1 Hennighausen
1   A 2 02-09-07_Hennighausen_PK_Mouse430_2_M47 GSP-EG003.1 Hennighausen
2   A 3 02-09-07_Hennighausen_PK_Mouse430_2_M50 GSP-EG003.1 Hennighausen
3   B 4 06-10-08_Hennighausen_1PK_Mouse430_2_4038WT GSP-EG003.2 Hennighausen
4   B 5 06-10-08_Hennighausen_2PK_Mouse430_2_4060WT GSP-EG003.2 Hennighausen
5   B 6 06-10-08_Hennighausen_3PK_Mouse430_2_4063WT GSP-EG003.2 Hennighausen
6 The input data set contained 45101 genes        
7 The filtered output data set contains 417 genes        
8 36365 gene rows removed by Include where A-B T-test p-value (two tailed) <= 0.05        
9 8319 gene rows removed by Include where A-B Mean Difference >= 1        
10   A 1 02-09-07_Hennighausen_PK_Mouse430_2_M44 GSP-EG003.1 Hennighausen


This Web page was derived in collaboration with GSP consortium members.

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Revised: Mon Sep 28 13:56:56 EDT 2009