Principal Investigator Investigators Experiment Group ID (1) Experiment Group Summary of experiment (2) Purpose of experiment (2) Experimental design (2) Stimulation (6) Mouse (strain) or Human Cell lines / tissue sample Genes WT Genes KO Affy chip(s) platform Dates samples were run Sample Preparation (Lit. ref. is ok) (8) Purification procedure (e.g., FACS, Ab, etc.) (8) Journal ref. if published (ref. PubMed ID) or not yet In mAdb DB (user, project name if yes) (8) In GEO DB (ID if yes) In caArray DB (ID if yes) Data location (mAdb, Geo, DVD, FTP server, Web site, etc.) Additional documentation (file(s), etc.) Lothar Hennighausen Bing-Mei Zhu, Weiping Chen EG001 Ter119+ erythroid cells from mouse fetal liver cells of control and Stat5-null mice Determine some of the major up and down regulated genes dependent on Stat5. Especially iron metabolism-related genes were down regulated and resulted in impaired iron uptake into erythropoietic cells and then microcytic anemia. Determine Stat5-dependent genes in the Erythropoietic cells in Stat5-KO or WT mice. Isolated Ter119-positive fetal liver cells from three to five E14.5 embryos of the same genotype were combined, and total RNA was extracted using TRIzol reagent (Life Technologies) with two additional ethanol precipitations. Microarray analyses were performed using Affymetrix Mouse Genome 430 2.0 array GeneChips (Affymetrix). Up- and down-regulated genes were selected based on P values of <0.05 and fold changes of >1.5 or -1.5 as assessed by ANOVA using Partek Pro software (Partek). To determine specific pathways, gene pathway analysis was obtained using the statistically significant gene list (P<0.05) represented on the chip. mouse Erythroid STAT5 STAT5-null MOE430_2 6/8/2004 B-M Zhu et.al. Blood, 2008 112: 2071-2080 B-M Zhu et.al. Blood, 2008 112: 2071-2081 B-M Zhu et.al. Blood, 2008 112: 2071-2080 GSE11777 No EDGE server Lothar Hennighausen Peter Klover, Weiping Chen EG003.1 Muscle cells from control and Stat5-null 12-week mice with GH treatment With GT treatment: three Stat5 fl/fl controls (#44, #47, and #50) and three Stat5 muscle-ko 12-week mice (Stat5MKO, #42, #43, and #48). Determine Stat5-dependent genes in the skeletal muscle with and without exogenous GH stimulation. The treated mice were given growth hormone (GH) midday for 3 hours and quadriceps muscle was isolated RNA and frozen in dry ice. RNA was isolated using TriZol reagent (Invitrogen) followed by RNeasy column purification(Qiagen). The untreated mice quadriceps muscle was isolated midday and frozen in dry ice. RNA was prepared as before. GH mouse Muscle STAT5 STAT5-null MOE430_2 2/9/2007 see Exper. Design see Exper. Design not yet GSE14710 No EDGE server Lothar Hennighausen Peter Klover, Weiping Chen EG003.2 Muscle cells from control and Stat5-null 15-week mice without GH treatment Without GH treatment: experiment three Stat5 fl/fl controls (#4035, #4036, and #4042) and three Stat5MKO (#4038, #4060, and #4063) 15 week mice . Determine Stat5-dependent genes in the skeletal muscle with and without exogenous GH stimulation. The treated mice were given growth hormone (GH) midday for 3 hours and quadriceps muscle was isolated RNA and frozen in dry ice. RNA was isolated using TriZol reagent (Invitrogen) followed by RNeasy column purification(Qiagen). The untreated mice quadriceps muscle was isolated midday and frozen in dry ice. RNA was prepared as before. GH mouse Muscle STAT5 STAT5-null MOE430_2 6/10/2008 see Exper. Design see Exper. Design not yet GSE14710 No EDGE server