A 1 06-10-08_Hennighausen_1PK_Mouse430_2_4038WT GSP-EG003.2 Hennighausen A 2 06-10-08_Hennighausen_2PK_Mouse430_2_4060WT GSP-EG003.2 Hennighausen A 3 06-10-08_Hennighausen_3PK_Mouse430_2_4063WT GSP-EG003.2 Hennighausen B 4 06-10-08_Hennighausen_4PK_Mouse430_2_4035KO GSP-EG003.2 Hennighausen B 5 06-10-08_Hennighausen_5PK_Mouse430_2_4036KO GSP-EG003.2 Hennighausen B 6 06-10-08_Hennighausen_6PK_Mouse430_2_4042KO GSP-EG003.2 Hennighausen The input data set contained 45101 genes The filtered output data set contains 19 genes 43442 gene rows removed by Include where A-B T-test p-value (two tailed) <= 0.05 1640 gene rows removed by Include where A-B Mean Difference <= -1 A A A B B B 06-10-08_Hennighausen_1PK_Mouse430_2_4038WT GSP-EG003.2 Hennighausen 06-10-08_Hennighausen_2PK_Mouse430_2_4060WT GSP-EG003.2 Hennighausen 06-10-08_Hennighausen_3PK_Mouse430_2_4063WT GSP-EG003.2 Hennighausen 06-10-08_Hennighausen_4PK_Mouse430_2_4035KO GSP-EG003.2 Hennighausen 06-10-08_Hennighausen_5PK_Mouse430_2_4036KO GSP-EG003.2 Hennighausen 06-10-08_Hennighausen_6PK_Mouse430_2_4042KO GSP-EG003.2 Hennighausen A-B p-Value A-B Mean Difference A Mean B Mean Well ID Feature ID Map mgB37_Probe Chr:Start-Stop mgB37_Probe Cytoband mgB37_RefSeq Chr:Start-Stop mgB37_RefSeq Cytoband Annotation_Src UniGene RefSeq Refseqs_Hit geneIDS_Hit Entrez GeneID Locus Tag Gene Description BioCarta Pathways KEGG Pathways Gene Ontology Terms GO Tier2 Component GO Tier3 Component GO Tier2 Function GO Tier3 Function GO Tier2 Process GO Tier3 Process 1091.3115 612.3186 635.9214 1565.4321 2320.3607 1921.4254 0.019383957 -1.34592 9.55423 10.90015 1327963 1416273_at 12 56.0 cM chr12:112692639-112692973 12 F1 chr12:112683139-112693229 12 F1 RSQ Mm.255332 NM_009396 1 1 21928 Tnfaip2 tumor necrosis factor, alpha-induced protein 2 (Tnfaip2), mRNA. angiogenesis;; cell differentiation;; multicellular organismal development anatomical structure formation;; cellular process;; developmental process;; multicellular organismal process anatomical structure development;; anatomical structure formation involved in morphogenesis;; anatomical structure morphogenesis;; cellular developmental process;; multicellular organismal development 74.1940 72.3079 66.3027 182.2095 154.0949 160.2428 0.00034890631 -1.22031 6.14677 7.36708 1327998 1416318_at 13 12.0 cM chr13:32934621-32937204 13 A3.2 chr13:32933960-32943054 13 A3.2 RSQ Mm.20144 NM_025429 1 1 66222 RP23-427P14.4 Serpinb1a serine (or cysteine) peptidase inhibitor, clade B, member 1a (Serpinb1a), mRNA. cytoplasm;; endopeptidase inhibitor activity;; regulation of protein catabolic process;; serine-type endopeptidase inhibitor activity cell;; cell part cell part;; intracellular;; intracellular part enzyme regulator activity enzyme inhibitor activity biological regulation;; metabolic process;; regulation of biological process catabolic process;; macromolecule metabolic process;; primary metabolic process;; regulation of biological process;; regulation of metabolic process 269.9078 781.6517 228.4423 2008.4111 1945.6615 2428.3396 0.040945566 -2.54042 8.50746 11.04788 1329660 1418370_at 14 10.0 cM chr14:32023339-32024836 14 B chr14:32021497-32024897 14 B RSQ Mm.439921 NM_009393 1 1 21924 Tnnc1 troponin C, cardiac/slow skeletal (Tnnc1), mRNA. Calcium signaling pathway;; Cardiac muscle contraction CLASS Environmental Information Processing; Signal Transduction; Calcium signaling pathway [PATH:mmu04020] Cellular Processes; Circulatory System; Cardiac muscle contraction [PATH:mmu04260] actin filament binding;; calcium ion binding;; regulation of ATPase activity;; regulation of muscle filament sliding speed binding ion binding;; protein binding biological regulation;; cellular process;; establishment of localization;; localization;; multicellular organismal process;; regulation of biological process actin filament-based process;; cellular localization;; establishment of localization;; establishment of localization in cell;; regulation of biological process;; regulation of biological quality;; regulation of cellular process;; regulation of localization;; regulation of molecular function;; regulation of multicellular organismal process;; system process;; transport 30.1154 38.2075 40.1069 111.3767 89.7235 88.2856 0.0011928135 -1.41894 5.16466 6.58361 1330250 1419086_at 5 chr5:44370129-44370679 5 B3 chr5:44370098-44373001 5 B3 RSQ Mm.46053 NM_008009 1 1 14181 Fgfbp1 fibroblast growth factor binding protein 1 (Fgfbp1), mRNA. extracellular region;; growth factor binding;; membrane;; plasma membrane cell;; cell part;; extracellular region cell part;; membrane binding protein binding 178.8438 211.0753 231.5755 587.5316 395.1282 487.1988 0.0054222097 -1.23119 7.68650 8.91769 1331506 1420884_at 9 chr9:53698088-53701597 9 A5.3 chr9:53698057-53701656 9 A5.3 RSQ Mm.29132 NM_025540 1 1 66402 Sln sarcolipin (Sln), mRNA. enzyme regulator activity;; integral to membrane;; membrane;; sarcoplasmic reticulum cell;; cell part;; organelle cell part;; intracellular;; intracellular organelle;; intracellular part;; membrane;; membrane part;; membrane-bounded organelle enzyme regulator activity 113.1034 81.5462 77.9860 162.8186 218.5802 179.4852 0.0093021397 -1.05022 6.48540 7.53562 1332186 1421830_at 4 47.6 cM chr4:101139016-101139266 4 C6 chr4:101092363-101139600 4 C6 RSQ Mm.42040 NM_009647 2 2 11639 RP23-301M3.3 Ak3l1 adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA. Metabolic pathways CLASS Metabolism; Nucleotide Metabolism; Purine metabolism [PATH:mmu00230];; Purine metabolism adenylate kinase activity;; ATP binding;; GTP binding;; kinase activity;; mitochondrion;; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process;; nucleobase, nucleoside, nucleotide kinase activity;; nucleotide binding;; nucleotide kinase activity;; phosphotransferase activity, phosphate group as acceptor;; transferase activity cell;; cell part;; organelle cell part;; intracellular;; intracellular organelle;; intracellular part;; membrane-bounded organelle binding;; catalytic activity nucleoside binding;; nucleotide binding;; transferase activity cellular process;; metabolic process cellular metabolic process;; nitrogen compound metabolic process;; primary metabolic process 517.6892 585.7949 674.0960 1349.6060 1406.0643 1441.9590 0.0052749455 -1.24753 9.20233 10.44986 1332719 1422558_at 10 43.0 cM chr10:79721002-79722530 10 C1 chr10:79720895-79723713 10 C1 RSQ Mm.7329 NM_010255 1 1 14431 Gamt guanidinoacetate methyltransferase (Gamt), mRNA. Arginine and proline metabolism;; Glycine, serine and threonine metabolism;; Metabolic pathways CLASS Metabolism; Amino Acid Metabolism; Glycine, serine and threonine metabolism [PATH:mmu00260] Metabolism; Amino Acid Metabolism; Arginine and proline metabolism [PATH:mmu00330] creatine biosynthetic process;; guanidinoacetate N-methyltransferase activity;; methyltransferase activity;; organ morphogenesis;; regulation of multicellular organism growth;; spermatogenesis;; transferase activity catalytic activity transferase activity biological regulation;; cellular process;; developmental process;; growth;; metabolic process;; multicellular organismal process;; regulation of biological process;; reproduction;; reproductive process anatomical structure development;; anatomical structure morphogenesis;; biosynthetic process;; cellular metabolic process;; gamete generation;; multicellular organism growth;; multicellular organismal development;; nitrogen compound metabolic process;; primary metabolic process;; regulation of biological process;; regulation of growth;; regulation of multicellular organismal process;; reproductive process;; sexual reproduction 1112.3910 1549.3411 1482.3400 3852.3579 2425.2815 2524.0403 0.018573107 -1.06881 10.41685 11.48566 1335990 1427201_at 14 chr14:31692621-31694325 14 B chr14:31692442-31694796 14 B RSQ Mm.220895 NM_181390 1 1 66175 Mustn1 musculoskeletal, embryonic nuclear protein 1 (Mustn1), mRNA. nucleus cell;; cell part;; organelle cell part;; intracellular;; intracellular organelle;; intracellular part;; membrane-bounded organelle 1937.2358 1153.9852 1051.9975 2594.0235 3370.5477 2549.9669 0.040723537 -1.08163 10.37704 11.45867 1347930 1438855_x_at 12 56.0 cM chr12:112692993-112693192 12 F1 IPA Tnfaip2 tumor necrosis factor, alpha-induced protein 2 98.3841 328.7075 154.5816 769.0408 813.4041 721.9121 0.049555307 -2.16573 7.41775 9.58347 1348252 1448554_s_at 14 20.0 cM chr14:55590223-55591731 14 C3 chr14:55589524-55613386 14 C3 RSQ Mm.457983 NM_080728 2 2 140781 Myh7 myosin, heavy polypeptide 7, cardiac muscle, beta (Myh7), mRNA. Cardiac muscle contraction;; Tight junction CLASS Cellular Processes; Cell Communication; Tight junction [PATH:mmu04530] Cellular Processes; Circulatory System; Cardiac muscle contraction [PATH:mmu04260] actin binding;; ATP binding;; calmodulin binding;; cytoplasm;; motor activity;; myofibril;; myosin complex;; nucleotide binding;; striated muscle contraction;; striated muscle thick filament cell;; cell part;; macromolecular complex;; organelle;; organelle part cell part;; intracellular;; intracellular organelle;; intracellular organelle part;; intracellular part;; non-membrane-bounded organelle;; organelle part;; protein complex binding;; catalytic activity hydrolase activity;; nucleoside binding;; nucleotide binding;; protein binding multicellular organismal process system process 35.0984 23.1433 29.4871 67.2878 68.1489 56.9569 0.010746921 -1.14894 4.84929 5.99823 1339138 1448680_at 12 51.0 cM chr12:105133323-105135281 12 E chr12:105133135-105143160 12 E RSQ Mm.439694 NM_009245 2 2 20702 Serpina1c serine (or cysteine) peptidase inhibitor, clade A, member 1C (Serpina1c), mRNA. endopeptidase inhibitor activity;; extracellular region;; protein amino acid N-linked glycosylation;; protein binding;; response to cytokine stimulus;; response to peptide hormone stimulus;; serine-type endopeptidase inhibitor activity extracellular region binding;; enzyme regulator activity enzyme inhibitor activity;; protein binding cellular process;; metabolic process;; response to stimulus biosynthetic process;; cellular metabolic process;; macromolecule metabolic process;; primary metabolic process;; response to chemical stimulus;; response to endogenous stimulus 249.1013 748.7493 300.0638 1856.5197 1897.4333 2285.0254 0.035580778 -2.38939 8.57935 10.96874 1340138 1449997_at 3 F1 chr3:89892969-89896013 3 F1 UGA Mm.240839 59069 Tpm3 Tropomyosin 3, gamma, mRNA (cDNA clone MGC:36031 IMAGE:4502880) Cardiac muscle contraction;; Pathways in cancer;; Thyroid cancer CLASS Cellular Processes; Circulatory System; Cardiac muscle contraction [PATH:mmu04260] Human Diseases; Cancers; Pathways in cancer [PATH:mmu05200] Human Diseases; Cancers; Thyroid Cancer [PATH:mmu05216] actin binding;; cleavage furrow;; cortical cytoskeleton;; cytoplasm;; cytoskeleton;; filamentous actin;; growth cone;; neuron projection;; podosome cell;; cell part;; macromolecular complex;; organelle;; organelle part cell division site;; cell division site part;; cell part;; cell projection;; intracellular;; intracellular organelle;; intracellular organelle part;; intracellular part;; non-membrane-bounded organelle;; organelle part;; protein complex;; site of polarized growth binding protein binding 218.2075 134.3381 114.9569 315.7167 362.3709 375.1926 0.040083447 -1.22369 7.22808 8.45177 1348663 1450387_s_at 4 47.6 cM chr4:101139123-101139504 4 C6 chr4:101092363-101139600 4 C6 RSQ Mm.42040 NM_009647 2 2 11639 RP23-301M3.3 Ak3l1 adenylate kinase 3-like 1 (Ak3l1), nuclear gene encoding mitochondrial protein, mRNA. Metabolic pathways CLASS Metabolism; Nucleotide Metabolism; Purine metabolism [PATH:mmu00230];; Purine metabolism adenylate kinase activity;; ATP binding;; GTP binding;; kinase activity;; mitochondrion;; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process;; nucleobase, nucleoside, nucleotide kinase activity;; nucleotide binding;; nucleotide kinase activity;; phosphotransferase activity, phosphate group as acceptor;; transferase activity cell;; cell part;; organelle cell part;; intracellular;; intracellular organelle;; intracellular part;; membrane-bounded organelle binding;; catalytic activity nucleoside binding;; nucleotide binding;; transferase activity cellular process;; metabolic process cellular metabolic process;; nitrogen compound metabolic process;; primary metabolic process 146.8151 436.8152 198.0208 1158.1453 1100.0280 1474.7292 0.028790526 -2.40297 7.86608 10.26905 1348770 1450813_a_at 1 60.0 cM chr1:137696087-137707566 1 E4 RSQ Mm.44379 NM_001112702 2 1 21952 Tnni1 troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA. actin binding;; ventricular cardiac muscle morphogenesis binding protein binding developmental process;; multicellular organismal process anatomical structure development;; anatomical structure morphogenesis;; multicellular organismal development 563.5964 511.8688 736.6675 1215.0369 1560.5489 1229.2415 0.0053308228 -1.15172 9.22101 10.37272 1343080 1460700_at 11 60.5 cM chr11:100748149-100748711 11 D chr11:100748123-100800825 11 D RSQ Mm.249934 NM_011486 3 1 20848 RP23-279L23.5 Stat3 signal transducer and activator of transcription 3 (Stat3), transcript variant 3, mRNA. EGF Signaling Pathway;; Erk1/Erk2 Mapk Signaling pathway;; IL 6 signaling pathway;; IL22 Soluble Receptor Signaling Pathway;; PDGF Signaling Pathway;; Role of ERBB2 in Signal Transduction and Oncology;; Signaling of Hepatocyte Growth Factor Receptor;; Stat3 Signaling Pathway;; TPO Signaling Pathway Acute myeloid leukemia CLASS Environmental Information Processing; Signal Transduction; Jak-STAT signaling pathway [PATH:mmu04630] Cellular Processes; Endocrine System; Adipocytokine signaling pathway [PATH:mmu04920] Cellular Processes; Immune System; Chemokine signaling pathway [PATH:mmu04062] Human Diseases; Cancers; Pathways in cancer [PATH:mmu05200];; Adipocytokine signaling pathway;; Chemokine signaling pathway;; Jak-STAT signaling pathway;; Pancreatic cancer;; Pathways in cancer acute-phase response;; calcium ion binding;; cytokine-mediated signaling pathway;; cytoplasm;; DNA binding;; eating behavior;; eye photoreceptor cell differentiation;; glucose homeostasis;; JAK-STAT cascade involved in growth hormone signaling pathway;; nucleus;; plasma membrane;; positive regulation of transcription from RNA polymerase II promoter;; protein binding;; protein dimerization activity;; protein kinase binding;; regulation of multicellular organism growth;; regulation of transcription from RNA polymerase II promoter;; regulation of transcription, DNA-dependent;; sequence-specific DNA binding;; sexual reproduction;; signal transducer activity;; signal transduction;; temperature homeostasis;; transcription;; transcription activator activity;; transcription factor activity;; transcription from RNA polymerase II promoter cell;; cell part;; organelle cell part;; intracellular;; intracellular organelle;; intracellular part;; membrane;; membrane-bounded organelle binding;; molecular transducer activity;; transcription regulator activity ion binding;; nucleic acid binding;; protein binding;; signal transducer activity;; transcription activator activity;; transcription factor activity biological regulation;; cellular process;; developmental process;; growth;; metabolic process;; multicellular organismal process;; positive regulation of biological process;; regulation of biological process;; reproduction;; response to stimulus anatomical structure development;; anatomical structure morphogenesis;; behavior;; biosynthetic process;; cell communication;; cellular developmental process;; cellular metabolic process;; cellular response to stimulus;; macromolecule metabolic process;; multicellular organism growth;; multicellular organismal development;; nitrogen compound metabolic process;; positive regulation of biological process;; positive regulation of cellular process;; positive regulation of metabolic process;; primary metabolic process;; regulation of biological process;; regulation of biological quality;; regulation of cellular process;; regulation of growth;; regulation of metabolic process;; regulation of multicellular organismal process;; response to chemical stimulus;; response to endogenous stimulus;; response to external stimulus;; response to stress;; sexual reproduction;; temperature homeostasis 208.0928 181.2786 169.2994 576.1519 675.4474 1166.6011 0.016057694 -2.05051 7.53553 9.58603 1370826 1428420_a_at 12 chr12:112667670-112669350 12 F1 chr12:112655640-112669394 12 F1 RSQ Mm.248640 NM_028807 1 1 74190 1200009I06Rik RIKEN cDNA 1200009I06 gene (1200009I06Rik), mRNA. 210.8462 215.7630 218.0549 459.4518 757.4940 561.2626 0.020322115 -1.43316 7.74730 9.18046 1365310 1453119_at 2 chr2:19581695-19582189 2 A3 UGA Mm.470264 Transcribed locus 195.5695 138.2218 161.4077 393.0408 457.7161 322.8464 0.0037471724 -1.24487 7.35232 8.59718 1367914 1457102_at 18 chr18:68501510-68501988 18 E2 UGA Mm.95774 Transcribed locus 31.6866 34.7647 32.0440 106.5041 232.3178 226.1078 0.021004874 -2.43608 5.03578 7.47186 1368821 1458115_at 8 chr8:64401070-64401578 8 B3.1 UGA Mm.440931 Transcribed locus