Erythroid data analyzed

( 1. Experimental Data   2. Analyses   3. Further Analyses )

***EDIT *** Control and fetal mouse liver Ter119+ erythroid Stat5-null mice were used to determine some of the major up and down regulated genes dependent on Stat5. Especially iron metabolism-related genes were down regulated and resulted in impaired iron uptake into erythropoietic cells and then microcytic anemia. (Investigators: Bing-Mei Zhu, Lothar Hennighausen, Weiping Chen).

Expression groups used in the tests: EG001

1. Experimental data

A GSP inventory of the all of the experiments is available in a Experiment Groups table. You can scroll to the particular Experiment Group ID. For a discussion on the data definitions used in "genes list, gene expression, JTV viewer, Excel data" below, see the Data Analysis Methods Section 5. that also defines terms used in the reports.

Descriptions of experiments used in the tests
It compares two groups of samples as A/B with:

EG001
Erythropoietic Ter119+ cells from mouse fetal liver cells from Stat5-KO and WT mice.
(T1) Stat5KO vs WT
      (A) Samples[Stat5KO]: EG001-01, EG001-02, EG001-03
      (B) Samples[WT]: EG001-04, EG001-05, EG001-06

2. Analyses

These tests report the gene changes for the specific Expression Groups. The gene expression as seen across all samples within the same Affymetrix platform is shown below in the Further Analyses across all samples. The tests are numbered T1+ (T1-) etc. meaning the fold-change of gene expression went up (down) in test 1, etc.

Fold-change Tests-Intersection report for Erythroid Excel, Test-Intersection Web page. This summarizes the fold-changes found for all of these tests.

EG001
Stat5KO vs WT
Erythropoietic Ter119+ cells from mouse fetal liver cells from Stat5-KO and WT mice.
  (T1+) --- foldChanges > 2X and p-Value<0.05, genes list, genes expression, JTV viewer, Excel
  (T1-) --- 1/foldChanges < 1/2X and p-Value<0.05, genes list, genes expression, JTV viewer, Excel

3. Further Analyses - genes viewed across all samples

These tests report the gene exprsesion for the all samples in the database (within the same Affymetrix platform) that were determined with the Expression Group Analyses. The tests are numbered T1+ (T1-) etc. meaning the fold-change of gene expression went up (down) in test 1, etc.

[Note: this was done with the 143 samples in EG001 through EG012, and does not include EG009-EG011 - 1/28/2009.]

EG001
Stat5KO vs WT
Erythropoietic Ter119+ cells from mouse fetal liver cells from Stat5-KO and WT mice.
  (T1+) --- foldChanges > 2X genes expression, JTV viewer, Excel
  (T1-) --- 1/foldChanges < 1/2X genes expression, JTV viewer, Excel

Go to the Introduction for a discussion of this database. Additional information on this database is available through the Table Of Contents on the left side of this Web site.

This Web page was derived in collaboration with GSP consortium members.

Revised: Mon Sep 28 15:13:49 EDT 2009
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